A Qbd Driven Approach for Development and Validation of an RP-UHPLC Method for Simultaneous Estimation of Ofloxacin and Fluconazole: An Application to Pharmaceutical Analysis

A simple reverse-phase UHPLC (RP-UHPLC) analytical method was developed and validated according to International Council on Harmonization (ICH) guidelines for simultaneous estimation of ofloxacin (OFX) fluconazole (FCZ) using a Response surface aided central composite design (CCD, Design-Expert®, Version 11.1.2.0). The linearity, selectivity, sensitivity, accuracy, precision, limits detection quantification, solution stability robustness. OFX FCZ separation were achieved Agilent 1290 Infinity II system encompassed with Waters Atlantis® T3 (150 × 4.6 mm2 i.d., 3 µm particle size) column, water containing 0.1% v/v formic acid methanol as isocratic mobile phase. eluted at 4.1 10.5 min no interference the excipients used in formulation λmax 260 nm. envisaged determine total drug content, vitro release study ex-vivo corneal permeation conventional well novel delivery formulations. proposed is simple, cost-effective, dependable reproducible could be regular analysis bulk quality controls research pharmaceutical industries.